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Image Search Results
Journal: Endocrinology
Article Title: Aldose Reductase Mediates NLRP3 Inflammasome–Initiated Innate Immune Response in Hyperglycemia-Induced Thp1 Monocytes and Male Mice
doi: 10.1210/en.2017-00294
Figure Lengend Snippet: Inhibition of AR prevented inflammatory cytokines in the serum of STZ-induced diabetic mice. Mice were made diabetic as described in Methods. The diabetic mice were treated with fidarestat (10 mg/kg/d) for 1, 2, 3, and 4 days. At indicated time intervals, the levels of IL-1β, TNF-α, IL-1α, IFNγ, MIP-1α, MCP-1, MIP-2, IL-12(p70), IL-12(p40), and IL-13 were measured in the serum by using Milliplex MAP Kit mouse inflammatory cytokine/chemokine magnetic bead panel (Merck) along with Luminex xMAP detection method, per manufacturer’s protocol in a Millipore Multiplex system (Merck). ND, nondetectable levels. The bars represent the mean ± standard error of the mean (n ≥ 4). *P < 0.05 vs control; **P < 0.01 vs diabetic. Ctrl, control; Fid, fidarestat; ND, not detectable.
Article Snippet: At indicated time intervals, the levels of IL-1 β , TNF- α , IL-1 α , IFN γ , MIP-1 α , MCP-1, MIP-2, IL-12(p70), IL-12(p40), and IL-13 were measured in the serum by using
Techniques: Inhibition, Luminex, Multiplex Assay